Selective Amino Acid Labeling / Free and Protected Amino Acids
Selective Amino Acid-Type Labeling
Selective amino acid-type labeling is used to simplify crowded spectra in order to aid in spectral interpretation or to provide specific probes for dynamic and structural studies. This technique requires the addition of free labeled amino acids (~50->250 mg / L)
to either minimal or rich growth media prior to protein induction. The cells will generally utilize the supplemented amino acids for protein synthesis prior to undergoing the de novo synthesis of the target amino acids. Please see page 25 for a complete listing of
amino acids that may be used with either in vivo growth systems or cell-free protein expression methods.
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Free Amino Acids
CK Gas Products offers CIL’s comprehensive list of labeled free amino acids for use in a variety of NMR investigations. The large number of available labeling schemes will maximize flexibility involved in designing experiments to probe structure, dynamics and binding of biological macromolecules. A number of packaged sizes are available, including 25 mg and 50 mg sizes, which are convenient for use in cell-free protein expression.
Protected Amino Acids
CK Gas Products offers CIL’s comprehensive list of protected amino acids for use in preparing peptides for use in NMR investigations. The large number of available labeling schemes maximizes the flexibility involved in designing experiments to probe binding, structure, and dynamics of peptides alone or in the presence of small molecules, proteins or nucleic acids. CIL offers amino acids that have N-tert-butoxycarbonyl (t-BOC) protecting groups and / or fluorenylmethoxycarbonyl (FMOC) amino-protecting groups.
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