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ISOTOPES_BANNER-VivoExpression

 

In Vivo Protein ExpressionStable Isotopes for Structural Biomolecular NMR Catalogue.

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FAQS

  • How do I know which minimal media reagents to use to make a protein of a specific uniform labeling pattern?
  • What algal strain is used to create BioExpress® 1000?
    • Agmenelum quadriplicatum
  • What algal strain is used to create Celltone® Powder and Celtone® Complete media?
    • Chlorella vulgaris
  • What algal strain is used to create the Amino Acid Mixes product?
    • Agmenelum quadriplicatum
  • What algal strain is the whole cells product?
    • Agmenelum quadriplicatum

Application Notes

TOP TEN TIPS FOR PRODUCING 13C, 15N PROTEIN IN ABUNDANCE (APPLICATION NOTE 15)
CELL-FREE PROTEIN SYNTHESIS WITH 2H/15N/13C-LABELED AMINO ACIDS IN H2O FOR THE PRODUCTION OF PERDEUTERATED PROTEINS WITH 1H IN THE EXCHANGEABLE POSITIONS (APPLICATION NOTE 36)
UNIFORM ISOTOPE LABELING OF EUKARYOTIC PROTEINS IN METHYLOTROPHIC YEAST FOR HIGH-RESOLUTION NMR STUDIES – EXTENSION TO MEMBRANE PROTEINS (APPLICATION NOTE 26)
ISOTOPE LABELING OF ALANINE METHYL GROUPS ON A DEUTERATED BACKGROUND FOR NMR STUDIES OF HIGH-MOLECULAR-WEIGHT PROTEINS (APPLICATION NOTE 25)
EFFICIENT UNIFORM ISOTOPE LABELING OF PROTEINS EXPRESSED IN BACULOVIRUS-INFECTED INSECT CELLS USING BIOEXPRESS®2000 (APPLICATION NOTE 14)
SPECIFIC ISOTOPIC LABELING OF METHYL GROUPS HAS EXTENDED THE MOLECULAR WEIGHT FOR NMR STUDIES OF PROTEIN STRUCTURE AND DYNAMICS (APPLICATION NOTE 16)
EXPRESSION OPTIMIZATION USING BIOEXPRESS 2000 MEDIA (APPLICATION NOTE 20)
[2,3-13C,]-LABELED AROMATIC RESIDUES AS A MEANS TO IMPROVING SIGNAL INTENSITIES AND KICK-STARTING THE ASSIGNMENTS (APPLICATION NOTE 22)
PICHIA PASTORIS AS A EUKARYOTIC PROTEIN ISOTOPE-LABELING SYSTEM
ALANINE PROBES OF SUPRA-MOLECULAR STRUCTURE AND DYNAMICS
OPTIMIZATION OF BIOEXPRESS® SUPPLEMENTATION OF M9 CULTURES (APPLICATION NOTE 12)

In vivo Protein Expression

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Isotope Enquiries: (01455) 828957
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Newtown Grange Farm Business Park,
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LE9 9FL

Contact Details

Isotopes
Tel: (01455) 828957
E-mail: enquiries@ckisotopes.com

Gases & Equipment
Tel: (01455) 560210
E-mail: sales@ckgas.com

Office 6, Newtown Grange Farm Business Park, Newtown Unthank, Leicestershire, LE9 9FL

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