Stable Isotope Labeling of Amino Acids in Cell Culture. A popular and simple MS technique that using heavy amino acids to metabolically label the proteome of cultured cells in vivo
SILAM is a labeling method that employs heavy amino acids or 15N to metabolically label rodent tissues in vivo.
Synthesised peptides with or without heavy stable isotopes are invaluable for targeted single reaction monitoring (SRM) assays and absolute MS quantitation. CIL offers amino acids and resin for synthesising peptides in your laboratory.
Chemical tagging refers to labeling proteins or peptides in vitro. This inexpensive method is advantageous over metabolic labeling due to its ability to label human samples and its low cost. In addition, chemical tagging can quantify more than two samples in one MS experiment.
One of the simplest methods of labeling peptides in vitro is the incorporation of 18O during proteolytic digestion.
Expression of large amounts of unlabeled and labeled proteins in heterologous systems is an invaluable tool with numerous biological applications. CIL offers a variety of media for protein expression and kits for in vitro translation.
To help researchers establish a stable LC-MRM/MS platform for bottom-up quantitative proteomics, Cambridge Isotope Laboratories, Inc. is pleased to offer PeptiQuant™ kits from MRM Proteomics Inc. These kits include the PeptiQuant™ Monthly LC-MS Platform Performance Kit, the PeptiQuant™ Daily LC-MS Platform Performance Kit, and the PeptiQuant™ Workflow Performance Kit. These innovative products are used to assess and track LC-MS performance of the LC-MS platform in a proteomic workflow in order to highlight any possible issues affecting quantitation.
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